LOCUS       KT031982                4104 bp    DNA     linear   SYN 02-AUG-2015
DEFINITION  Synthetic plasmid Sp_opt-An_cas9 Cas9 (Cas9) gene, partial cds.
ACCESSION   KT031982
VERSION     KT031982.1
KEYWORDS    .
SOURCE      Synthetic plasmid Sp_opt-An_cas9
  ORGANISM  Synthetic plasmid Sp_opt-An_cas9
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 4104)
  AUTHORS   Nodvig,C.S., Nielsen,J.B., Kogle,M.E. and Mortensen,U.H.
  TITLE     A CRISPR-Cas9 System for Genetic Engineering of Filamentous Fungi
  JOURNAL   PLoS ONE 10 (7), E0133085 (2015)
   PUBMED   26177455
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4104)
  AUTHORS   Noedvig,C.S., Nielsen,J.B., Kogle,M.E. and Mortense,U.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JUN-2015) DTU Systems Biology, Technical University
            of Denmark, Soeltoftsplads, build. 223, Kgs. Lyngby 2800, Danmark
FEATURES             Location/Qualifiers
     source          1..4104
                     /organism="Synthetic plasmid Sp_opt-An_cas9"
                     /mol_type="other DNA"
                     /db_xref="taxon:1688604"
     gene            1..>4104
                     /gene="Cas9"
     CDS             1..>4104
                     /gene="Cas9"
                     /note="derived from Streptococcus pyogenes cas9; codon
                     optimized for Aspergillus niger"
                     /codon_start=1
                     /transl_table=11
                     /product="Cas9"
                     /protein_id="AKS40378.1"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIK
                     KNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLE
                     ESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAH
                     MIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSK
                     SRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLD
                     NLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTL
                     LKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVK
                     LNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPY
                     YVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVL
                     PKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKE
                     DYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLT
                     LFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDF
                     LKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQT
                     VKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKE
                     HPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKV
                     LTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKA
                     GFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQF
                     YKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIG
                     KATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLS
                     MPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVV
                     AKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLF
                     ELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQH
                     KHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGA
                     PAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD"
ORIGIN      
        1 atggacaaga agtatagcat cgggctggac attggaacga actcggttgg ttgggctgtg
       61 attacggacg aatacaaggt gccatccaag aagtttaagg tcctgggaaa caccgaccgt
      121 cactcaatca agaagaatct cattggagcc ctgctcttcg atagtgggga gaccgccgaa
      181 gctactcgac tgaagcgaac ggctcgccgg cgttatacac gacgcaagaa tcgcatctgc
      241 tacctccagg agattttcag caacgaaatg gctaaggttg atgactcatt ctttcatcga
      301 ctcgaagaaa gtttcttggt cgaggaggat aagaagcacg agcgccatcc gatctttggt
      361 aacattgtgg atgaggttgc ctatcacgaa aagtacccaa ctatctatca tcttcgtaag
      421 aagctggtcg atagcacgga caaggctgat ttgcgactta tctacctggc actcgcgcac
      481 atgattaagt tccgcggcca ttttcttatc gagggtgacc tgaaccccga taattctgac
      541 gttgataagc tcttcatcca gttggtccaa acctacaatc agctgtttga ggaaaaccct
      601 attaatgcat ctggcgtgga cgccaaggct atcctttcgg cgcgcctgtc taagtcgcgg
      661 cgtttggaga accttatcgc acaactcccc ggcgaaaaga agaacggcct cttcggtaat
      721 ttgattgcgt tgtcacttgg tctgactcct aacttcaaga gtaattttga cctggcagag
      781 gatgcgaagc tccagttgtc taaggatacg tatgatgacg atctcgacaa cttgcttgcc
      841 caaatcggtg accagtacgc tgatcttttc ctggccgcta agaatctctc agatgcaatc
      901 ctgctcagtg acattttgcg ggtcaacacc gagattacta aggcccccct gtcagctagt
      961 atgatcaagc ggtatgatga gcaccatcag gacctcacct tgcttaaggc cctcgtgcgt
     1021 cagcaattgc ctgagaagta caaggaaatc ttctttgacc aatccaagaa cggatacgca
     1081 gggtatattg atggcggtgc gagccaggag gaattctaca agtttatcaa gccgattttg
     1141 gagaagatgg acggcactga ggaactgctc gtcaagctga atcgcgaaga tttgcttcgt
     1201 aagcaacgaa cgttcgacaa cggctccatc ccgcaccaga ttcatctggg cgagctccac
     1261 gccatccttc gacgccagga agatttctac ccatttctga aggacaaccg tgagaagatc
     1321 gaaaagattc ttacattccg aatcccctac tatgtgggac ctttggcccg tgggaattcc
     1381 cgatttgctt ggatgacccg aaagagcgag gaaaccatca ctccgtggaa cttcgaggaa
     1441 gtcgtggaca agggtgcatc cgcgcagagc ttcattgagc ggatgaccaa ttttgataag
     1501 aaccttccga atgaaaaggt cctgccaaag cattcgctgc tctacgagta tttcaccgtg
     1561 tataacgaac tgactaaggt caagtacgtg acggagggaa tgcggaagcc agccttcctc
     1621 tcaggggaac aaaagaaggc tatcgtcgat ttgcttttta agaccaatcg taaagtgact
     1681 gttaagcagc tgaaggagga ttatttcaag aagattgaat gtttcgactc cgtcgagatc
     1741 agcggcgtgg aagatcgctt taacgcttcc ctcggtacct accacgacct gctcaagatc
     1801 attaaggaca aggatttcct cgataacgag gaaaatgagg acatcttgga agatattgtc
     1861 ctcacgttga cactttttga ggaccgcgaa atgatcgagg aacggctcaa gacatatgcc
     1921 catttgttcg acgataaggt gatgaagcag ctgaagcggc gtcgatacac cggatggggt
     1981 cgccttagcc ggaagctgat caacggcatt cgagataagc aatctggtaa gactatcttg
     2041 gatttcctta agtcggacgg cttcgccaac cgcaatttta tgcagcttat tcacgacgat
     2101 tccctgacgt tcaaggagga catccagaag gcacaagtct caggacaagg ggattccctg
     2161 cacgagcata tcgccaacct ggctggatcc ccggcgatca agaaggggat tcttcagacc
     2221 gtcaaggttg tcgacgagct ggtcaaggtg atgggccgtc ataagccaga aaacatcgtg
     2281 attgagatgg cccgagaaaa tcagaccact caaaagggtc agaagaacag ccgcgagcgg
     2341 atgaagcgga tcgaggaagg cattaaggaa cttggttctc agatcctgaa ggagcaccct
     2401 gttgaaaaca cacagctcca aaatgagaag ctgtatctct actatttgca aaatggacgc
     2461 gacatgtacg tcgatcagga gctcgacatt aaccggttgt cggactacga tgttgaccat
     2521 atcgtcccgc aatccttcct taaggacgat agcattgata acaaggtgct gactcgctca
     2581 gataagaacc ggggcaagtc cgacaatgtt ccaagcgagg aagtggttaa gaagatgaag
     2641 aactactggc gccaattgct taatgccaag ctcatcacac agcgcaagtt tgacaacttg
     2701 accaaggccg agcggggagg gctgagtgaa ctcgataagg ctggcttcat caagcgtcaa
     2761 ctcgtggaga cgcgacagat cacaaagcac gttgctcaga ttctggactc ccggatgaac
     2821 acaaagtacg acgagaatga taagctcatc cgtgaagtta aggtcattac cctcaagtct
     2881 aagttggtgt cggatttccg caaggacttc caattttata aggttcggga gatcaacaat
     2941 tatcaccatg cacatgatgc gtacctcaac gcagtcgtgg gaactgcgct catcaagaag
     3001 tatcccaagt tggagtccga attcgtctac ggggattata aggtttacga cgtccgcaag
     3061 atgatcgcca agagtgagca ggaaattggc aaggccacgg ctaagtattt cttttactcc
     3121 aacatcatga atttctttaa gacggagatc acactcgcca atggagaaat ccgtaagcga
     3181 cctttgattg agaccaacgg cgagactggt gaaatcgttt gggataaggg gcgcgacttc
     3241 gctaccgtgc ggaaggttct gagcatgccg caagtcaata tcgtcaagaa aaccgaggtg
     3301 cagacaggcg gtttctctaa ggaatcgatt cttccaaagc gtaactctga caagctgatc
     3361 gctcgaaaga aggattggga ccccaagaag tatggagggt tcgattctcc tacagtggca
     3421 tactcggttc tcgttgtcgc gaaggttgag aagggaaagt ctaagaagct gaagtcggtc
     3481 aaggaactgc tcgggatcac cattatggag cgctccagct tcgaaaagaa tcccatcgac
     3541 tttctcgagg ccaagggcta taaggaagtc aagaaggatc ttatcattaa gctgcctaag
     3601 tactctttgt tcgagcttga aaacggtcga aagcgaatgc tcgcatcggc aggagagttg
     3661 cagaagggga atgaattggc acttccctca aagtacgtga acttcctgta tctcgcgtcc
     3721 cactacgaga agctgaaggg tagccctgag gacaacgaac agaagcaact ttttgttgag
     3781 caacacaagc attatctgga tgagatcatt gaacagattt cagagttcag taagcgcgtc
     3841 atcctcgccg atgctaatct cgacaaggtg ttgtcggcct acaacaagca ccgtgacaag
     3901 ccgatccgag agcaggctga aaatatcatt catctgttca ccctcactaa cttgggagca
     3961 ccagcagcgt tcaagtattt tgatacgaca atcgaccgta agcgatacac gtccacaaag
     4021 gaggtgcttg atgcgaccct gattcatcaa tccatcactg ggctctatga aacccgtatc
     4081 gaccttagtc aactgggggg cgac
//